In the present study, we review the status of the genus Mastigusa Menge, 1854 in Bulgaria. We identified two species from the country, namely M. arietina (thorell, 1871) and M. macrophthalma (kulczyński, 1897). this is the first record of M. arietina from Bulgaria. Furthermore, we report a male palpal polymorphism in M. arietina in the populations around the Black Sea. We also provide molecular support for our conclusions, combining newly generated DNA sequences with information available in genBank.