In the present study, we review the status of the genus Mastigusa Menge, 1854 in Bulgaria. We identified two species from the country, namely
Mastigusa arietina (thorell, 1871) and M. macrophthalma (kulczyński, 1897). this is the first record of
Mastigusa arietina from Bulgaria. Furthermore, we report a male palpal polymorphism in
Mastigusa arietina in the populations around the Black Sea. We also provide molecular support for our conclusions, combining newly generated DNA sequences with information available in genBank.